日本黄A级A片国产免费,黄色视频在线免费看,亚洲国产精品一区二区久久阿宾,久久国产精品99久久人人澡,另类TS人妖一区二区三区

Time resolved fluorescence immunoassay technology opens a new era of ipoct intelligent detection!

2017-06-02

The annual big bang of products falls on Qingdao this year. As expected, POCT manufacturers alone reached an unprecedented scale at this inspection medicine and blood transfusion instrument and reagent Expo, and Ruihui biology ranked among the top ten "IVD enterprises with the most craftsmanship spirit in China".
With the rapid development of science and technology, the detection sensitivity of many markers needs to reach pg / ml. the first generation POCT products based on colloidal gold chromatography still can not meet the requirements of sensitivity by increasing the amount of samples and prolonging the reaction time. Now they have gradually withdrawn from the historical dance stage. Instead, ordinary fluorescence immunochromatography products have improved their sensitivity, but ordinary fluorescence immunochromatography takes fluorescein as a marker. The results cannot be eliminated due to the influence of stray light, and the quantification is not accurate. The latest generation of POCT products came into being. Time-resolved fluorescence immunochromatography technology takes fluorescent microspheres as markers, with high sensitivity, no stray light interference and more accurate quantification.

1. Marker selection: fluorescein and fluorescent microspheres


Fluorescein is a small molecular compound with molecular weight of hundreds to thousands. It is crosslinked with antibody (antigen) through active groups, and produces fluorescence effect in final use for quantification. There are many kinds of fluorescein, and the excitation range almost covers the ultraviolet to infrared region. However, the influence of stray light cannot be eliminated and the quantification is inaccurate. As shown below:

 

 

Fluorescent microspheres are made by filling nano polystyrene microspheres with lanthanide elements (the most commonly used is europium EU). After crosslinking with antibody (antigen), the microspheres can be used for antigen (antibody) detection. Fluorescent microspheres have greater stock displacement than fluorescein. For example, the most common EU, the excitation light is 365nm, while the emission light is about 610nm, and the stock displacement is greater than 200nm. It can effectively eliminate the interference of excitation light and nonspecific fluorescence on signal acquisition and improve the specificity of test. EU is an inert element. Compared with fluorescein, it is not easy to be bleached by light, and the result is more stable.

 

Fluorescent microspheres have a long fluorescence attenuation time (500 ~ 2000us), while the fluorescence signal attenuation time of fluorescein is very short (5 ~ 100US). Based on this, fluorescent microspheres can develop time-resolved technology. The measurement time can be appropriately delayed and measured after the background fluorescence is completely attenuated, which can eliminate the interference of excitation and improve the accuracy of inspection.

 

Based on the above two points, it is not too much to say that the time-resolved fluorescence of nano microspheres has opened a new decade of POCT marker detection technology.

 

 

Comparison diagram of time-resolved fluorescence and ordinary fluorescence

 

 

2. Operation simplification: one-step and two-step


The so-called one step and two steps refer to the operation steps.


The one-step method simplifies the personnel operation by adding the sample to the reaction plate, which basically meets the performance requirements (mainly precision and accuracy) and reduces the operation intensity for low sensitivity projects (such as MAU). However, for heart markers, which are highly sensitive and valuable for the diagnosis of acute cardiovascular diseases, the one-step method of changing operation for performance is embarrassing.


Compared with the one-step method, the two-step method dilutes the sample before adding the reaction plate to effectively eliminate the interference of impurities in the sample, so as to improve the precision and accuracy, and ensure that the test results will not mislead patients for treatment due to insufficient performance.

 

3. Antigen antibody


Fawalski, a lifelong academician of Russian aeroengine, said: "all things on aircraft increase resistance and weight, but the engine improves power. As long as the engine is good, it can fly with a piece of wood."


This principle also applies to products based on antigen antibody reaction. Good antigen antibody is like a good engine. In any case, high-quality and stable biological raw materials should be selected. The quality of antigen antibody accounts for at least 50% of the product quality. Therefore, the antigens and antibodies used in Ruihui biological products come from internationally renowned manufacturers, and the stable product quality is ensured through strict quality control and matching.

 

4. Spring of domestic equipment


In January 2017, the notice of Sichuan Provincial Department of Finance on publishing the list of imported products purchased by provincial governments in 2017, which was posted on the Sichuan provincial government procurement network, explicitly required that hospitals (or units) with grade II and below should use domestic products of 93 kinds of medical equipment; At the national level, Zhejiang, Shanghai, Jiangsu, Guangdong and Sichuan are included in the first batch of pilot promotion of domestic medical equipment.


In March 2017, China Medical Equipment Association released the third batch of excellent domestic medical equipment product catalogue, and POCT products entered the catalogue of excellent domestic medical equipment for the first time.


The coincidence of the government and the association seems to be calling the world tomorrow. The spring of domestic equipment is not far away, especially for time-resolved fluorescence immunochromatography. Ruihui biological seized the dividend given by the times, continuously improved the comprehensive strength of products, raised POCT time-resolved immunochromatography technology to a new level, focused on precision medicine and opened a new era of ipoct intelligent detection!

 

點(diǎn)擊咨詢

仙踪林一级婬片A片 | 国产又大又黄的视频 | 成人海角社区在线观看 | 无码人妻精品一区二区蜜桃网站文 | 浙江妇搡BBBB搡BBBB | 特黄特色的大片免费视频 | 狠狠人妻久久久久久综合蜜桃 | 国产成人无码精品色欲天香 | 欧美猛交XXXXX无码 | 国产 成年妇 视频 麻豆 视频免费网站黄 | 人人操人人妻人人爱人人人DVD | 黄色视频免费在线观看 | 少妇被大狼狗躁A片无码免费 | 国产呦小泬泬99精品 | 天天影视色欲综合插插插 | 精品国产AⅤ一区二区三区东京热 | 精品人妻无码一区二区 | 欧美人与性动交A级毛片 | 妖精视频在线观看国产一区无码 | 色欲av久久人妻蜜臀免费网站 | 国产无套内谢普通话对白天美传媒 | 精品一区二区三区呻吟声 | 高潮又爽又黄又无遮挡高清 | 精品国婬伦v无码久久久黑人 | 国产第一A片成人网站777 | 国产精品国产三级国芦专播精品人 | 精品国婬伦v无码久久久黑人 | 国产三级片网站在线观看 | 看黄色一级免费的黄色视频 | 亚洲无码不卡毛片在线看 | 紧身裤蜜桃臀久久影院 | 国产精品爆乳在线第一区 | 人妻无码一区二区三区久 | 国内少妇人妻偷人精品四虎 | 国产色情a 6 2v久久无码无码 | 伊人中文字幕在线观看 | 国产午夜精品人妻网站 | 精品99视频免费在线观看 | 国产毛片精品区色欲黄A片 州产精无码久久久久久高潮 | 亚洲中文久久精品无码比基尼 | 欧美三级午夜理伦三级 |